1. Inhalation depth error
If the liquid depth in the 8-channel pipetting workstation is correct, the accuracy can be improved by 5%. According to the size of the suction head, the suction depth of the micropipette workstation should be 1-2 mm, and the suction depth of the large-scale pipette workstation should be 3-6 mm. If the suction head enters the liquid too deeply, the air pressure in the suction head will increase, leading to excessive liquid suction.
2. Incorrect liquid transfer angle
The inlet angle of the pipette in the sample should be as close as possible to 90 degrees, and the deviation from the vertical direction should not exceed 20 degrees. Excessive angle may cause the suction head to inhale too much liquid, resulting in inaccurate suction. For example, when at an angle of 30 degrees from the vertical, up to 0.7% of the liquid will be excessively sucked in.
3. Inconsistent liquid separation
By ensuring complete separation of remaining droplets rather than adhering to the front end of the suction head, the accuracy of the product and reproducibility between samples can be achieved. For most applications, it is recommended to use the front end of the suction head to separate the liquid along the container wall, as this can reduce or eliminate the amount of sample left in the suction head. This technology can increase accuracy by 1% or higher.
4. Wetting error
5. Inconsistent rhythm of liquid transportation